Just Open your image file with Separation Studio, and it will do the rest. You don't need any other software to separate your graphics colors. Separation Studio is a color separation tool with Single Color and CMYK color separation type. The separations can be based on shapes defined in the application. Separation Studio is an application that allows you to create single color and cmyk half-tone patterns in vector- based formats. Separation Studio is powerful, easy to use color separation and artwork generator tool designed exclusively for Mac.